Year 2020-2017

Publications

2020

1. Cell-Cycle-Associated Expression Patterns Predict Gene Function in Mycobacteria. Bandekar AC, Subedi S, Ioerger TR, Sassetti CM. Curr Biol. 2020 Oct 19;30(20):3961-3971.e6. doi: 10.1016/j.cub.2020.07.070. Epub 2020 Sep 10. PMID: 32916109 

2. Distinct Bacterial Pathways Influence the Efficacy of Antibiotics against Mycobacterium tuberculosis. Bellerose MM, Proulx MK, Smith CM, Baker RE, Ioerger TR, Sassetti CM. mSystems. 2020 Aug 4;5(4):e00396-20. doi: 10.1128/mSystems.00396-20. PMID: 32753506

3. Pyrazinamide triggers degradation of its target aspartate decarboxylase. Gopal P, Sarathy JP, Yee M, Ragunathan P, Shin J, Bhushan S, Zhu J, Akopian T, Kandror O, Lim TK, Gengenbacher M, Lin Q, Rubin EJ, Grüber G, Dick T. Nat Commun. 2020 Apr 3;11(1):1661. doi: 10.1038/s41467-020-15516-1. PMID: 32245967. PMCID: PMC7125159.

4. Structural insights into phosphopantetheinyl hydrolase PptH from Mycobacterium tuberculosis. Mosior J, Bourland R, Soma S, Nathan C, Sacchettini J. Protein Sci. 2020 Mar;29(3):744-757. doi: 10.1002/pro.3813. Epub 2020 Jan 20. PMID: 31886928

5. The molecular basis of pyrazinamide activity on Mycobacterium tuberculosis PanD. Sun Q, Li X, Perez LM, Shi W, Zhang Y, Sacchettini JC. Nat Commun. 2020 Jan 17;11(1):339. doi: 10.1038/s41467-019-14238-3. PMID: 31953389 

6. The conserved translation factor LepA is required for optimal synthesis of a porin family in Mycobacterium smegmatis. Fishbein SRS, Tomasi FG, Wolf ID, Dulberger CL, Wang A, Keshishian H, Wallace L, Carr SA, Ioerger TR, Rego EH, Rubin EJ. J Bacteriol. 2020 Dec 23;203(6):e00604-20. doi: 10.1128/JB.00604-20. Online ahead of print. PMID: 33361193. PMCID: PMC8095456.

7. Hierarchical natural move Monte Carlo refines flexible RNA structures into cryo-EM densities. Chang JY, Cui Z, Yang K, Huang J, Minary P, Zhang J. RNA. 2020 Dec;26(12):1755-1766. doi: 10.1261/rna.071100.119. Pub 2020 Aug 21. PMID: 32826323

2019

8. Iron Acquisition in Mycobacterium tuberculosis. Chao A, Sieminski PJ, Owens CP, Goulding CW. Chem Rev. 2019 Jan 23;119(2):1193-1220. doi: 10.1021/acs.chemrev.8b00285. Epub 2018 Nov 26. PMID: 30474981

9. Infect and Inject: How Mycobacterium tuberculosis Exploits Its Major Virulence-Associated Type VII Secretion System, ESX-1. Tiwari S, Casey R, Goulding CW, Hingley-Wilson S, Jacobs WR Jr. Microbiol Spectr. 2019 May;7(3):10.1128/microbiolspec.BAI-0024-2019. doi: 10.1128/microbiolspec.BAI-0024-2019. PMID: 31172908 

10. Structure-Guided Drug Design of 6-Substituted Adenosine Analogues as Potent Inhibitors of Mycobacterium tuberculosis Adenosine Kinase. Crespo RA, Dang Q, Zhou NE, Guthrie LM, Snavely TC, Dong W, Loesch KA, Suzuki T, You L, Wang W, O’Malley T, Parish T, Olsen DB, Sacchettini JC. J Med Chem. 2019 May 9;62(9):4483-4499. doi: 10.1021/acs.jmedchem.9b00020. Epub 2019 Apr 19. PMID: 31002508 

11. Selection and characterization of ultrahigh potency designed ankyrin repeat protein inhibitors of C. difficile toxin B. Simeon R, Jiang M, Chamoun-Emanuelli AM, Yu H, Zhang Y, Meng R, Peng Z, Jakana J, Zhang J, Feng H, Chen Z. PLoS Biol. 2019 Jun 24;17(6):e3000311. doi: 10.1371/journal.pbio.3000311. eCollection 2019 Jun. PMID: 31233493 

12. Two Accessory Proteins Govern MmpL3 Mycolic Acid Transport in Mycobacteria. Fay A, Czudnochowski N, Rock JM, Johnson JR, Krogan NJ, Rosenberg O, Glickman MS. mBio. 2019 Jun 25;10(3):e00850-19. doi: 10.1128/mBio.00850-19. PMID: 31239378

13. Opposing reactions in coenzyme A metabolism sensitize Mycobacterium tuberculosis to enzyme inhibition. Ballinger E, Mosior J, Hartman T, Burns-Huang K, Gold B, Morris R, Goullieux L, Blanc I, Vaubourgeix J, Lagrange S, Fraisse L, Sans S, Couturier C, Bacqué E, Rhee K, Scarry SM, Aubé J, Yang G, Ouerfelli O, Schnappinger D, Ioerger TR, Engelhart CA, McConnell JA, McAulay K, Fay A, Roubert C, Sacchettini J, Nathan C. Science. 2019 Feb 1;363(6426):eaau8959. doi: 10.1126/science.aau8959. PMID: 30705156

14. R pyocin tail fiber structure reveals a receptor-binding domain with a lectin fold. Salazar AJ, Sherekar M, Tsai J, Sacchettini JC. PLoS One. 2019 Feb 5;14(2):e0211432. doi: 10.1371/journal.pone.0211432. Collection 2019. PMID: 30721244 

15. A Single Mutation in the Mycobacterium tuberculosis Heme-Degrading Protein, MhuD, Results in Different Products. Chao A, Goulding CW. Biochemistry. 2019 Feb 12;58(6):489-492. doi: 0.1021/acs.biochem.8b01198. Epub 2019 Jan 9. PMID: 30605595 

16. Structural Variability of EspG Chaperones from Mycobacterial ESX-1, ESX-3, and ESX-5 Type VII Secretion Systems. Tuukkanen AT, Freire D, Chan S, Arbing MA, Reed RW, Evans TJ, Zenkeviciutė G, Kim J, Kahng S, Sawaya MR, Chaton CT, Wilmanns M, Eisenberg D, Parret AHA, Korotkov KV. J Mol Biol. 2019 Jan 18;431(2):289-307. doi: 10.1016/j.jmb.2018.11.003. Epub 2018 Nov 10. PMID: 30419243 

17. Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathway. Miller BK, Hughes R, Ligon LS, Rigel NW, Malik S, Anjuwon-Foster BR, Sacchettini JC, Braunstein M. Elife. 2019 Jan 3;8:e40063. doi: 10.7554/eLife.40063. PMID: 30604681

18. The structure of the endogenous ESX-3 secretion system. Poweleit N, Czudnochowski N, Nakagawa R, Trinidad DD, Murphy KC, Sassetti CM, Rosenberg OS. Elife. 2019 Dec 30;8:e52983. doi: 10.7554/eLife.52983. PMID: 31886769

19. Structure of a Mycobacterium tuberculosis Heme-Degrading Protein, MhuD, Variant in Complex with Its Product. Chao A, Burley KH, Sieminski PJ, de Miranda R, Chen X, Mobley DL, Goulding CW. Biochemistry. 2019 Nov 19;58(46):4610-4620. doi: 10.1021/acs.biochem.9b00726. Epub 2019 Nov 6. PMID: 31638374

20. Structural and functional insight into the Mycobacterium tuberculosis protein PrpR reveals a novel type of transcription factor. Tang S, Hicks ND, Cheng YS, Silva A, Fortune SM, Sacchettini JC. Nucleic Acids Res. 2019 Oct 10;47(18):9934-9949. doi: 10.1093/nar/gkz724. PMID: 31504787

21. A Multiscale Model for the Self-Assembly of Coat Proteins in Bacteriophage MS2. Wang B, Zhang J, Wu Y. J Chem Inf Model. 2019 Sep 23;59(9):3899-3909. doi: 10.1021/acs.jcim.9b00514. Epub 2019 Aug 23. PMID: 31411466

22. Structural basis for the adsorption of a single-stranded RNA bacteriophage. Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J. Nat Commun. 2019 Jul 16;10(1):3130. doi: 10.1038/s41467-019-11126-8. PMID: 31311931

2018-2017

23. TnSeq of Mycobacterium tuberculosis clinical isolates reveals strain-specific antibiotic liabilities. Carey AF, Rock JM, Krieger IV, Chase MR, Fernandez-Suarez M, Gagneux S, Sacchettini JC, Ioerger TR, Fortune SM. PLoS Pathog. 2018 Mar 5;14(3):e1006939. doi: 10.1371/journal.ppat.1006939. eCollection 2018 Mar. PMID: 29505613 

24. Anion-π Interactions in Computer-Aided Drug Design: Modeling the Inhibition of Malate Synthase by Phenyl-Diketo Acids. Ellenbarger JF, Krieger IV, Huang HL, Gómez-Coca S, Ioerger TR, Sacchettini JC, Wheeler SE, Dunbar KR. J Chem Inf Model. 2018 Oct 22;58(10):2085-2091. doi: 10.1021/acs.jcim.8b00417. Epub 2018 Sep 12. PMID: 30137983

25. The affinity of MhuD for heme is consistent with a heme degrading function in vivo. Thakuri B, Graves AB, Chao A, Johansen SL, Goulding CW, Liptak MD. Metallomics. 2018 Nov 14;10(11):1560-1563. doi: 10.1039/c8mt00238j. PMID: 30239544 

26. A Lysine Acetyltransferase Contributes to the Metabolic Adaptation to Hypoxia in Mycobacterium tuberculosis. Rittershaus ESC, Baek SH, Krieger IV, Nelson SJ, Cheng YS, Nambi S, Baker RE, Leszyk JD, Shaffer SA, Sacchettini JC, Sassetti CM. Cell Chem Biol. 2018 Dec 20;25(12):1495-1505.e3. doi: 10.1016/j.chembiol.2018.09.009. Epub 2018 Oct 11. PMID: 30318462 

27. Development of a novel lead that targets M.tuberculosis polyketide synthase 13. Aggarwal A, Parai MK, Shetty N, Wallis D, Woolhiser L, Hastings C, Dutta NK, Galaviz S, Dhakal RC, Shrestha R, Wakabayashi S, Walpole C, Matthews D, Floyd D, Scullion P, Riley J, Epemolu O, Norval S, Snavely T, Robertson GT, Rubin EJ, Ioerger TR, Sirgel FA, van der Merwe R, van Helden PD, Keller P, Böttger EC, Karakousis PC, Lenaerts AJ, Sacchettini JC. Cell. 2017 Jul 13;170(2):249-259.e25. doi: 10.1016/j.cell.2017.06.025. Epub 2017 Jun 29. PMID: 28669536; PMCID: PMC5509550.